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Theoretically develop a new analytical method for measuring AgNP concentrations intracellularly

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Must be 3-4 pages double spaced, at least one source cited and referenced in American Chemical Society format

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Chem 3030 Analytical II Instrumental Analysis

In Chem 3030, Analytical II Instrumental Lecture, students, by the completion of the course, will learn through lecture, assigned problems, and quizzes, the theory and practical real-world Chemistry Instrumental Analysis using the techniques described below. In addition, the students are individually assigned a Theoretical Project, which requires a detailed literature investigation and report.

In the beginning of the course the Analytical Process, Measurements and Calculations, and Treatment of Data, which includes: Experimental Error, Statistics, Quality Assurance, and Calibration Methods, will be covered to bring all of the students to a level mathematical playing field.    

Students will be immersed in the theory and practice of Sample Preparation, Analytical Separations, Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), Chromatography Method Development, Capillary Electrophoretic Techniques, and other special separation topics in real-life examples. Fundamentals of Spectrophotometry and its applications will be covered, which includes: the types and design of Spectrophotometers, Atomic Absorption Spectroscopy (AAS), Inductively Coupled Plasma Spectroscopy Optical Emission Spectroscopy (ICP-OES, and X-ray Fluorescence (XRF) Spectrometry in theory and their use in real-life scenarios.

The students will be introduced to Mass Spectrometry (MS), and its hyphenated techniques: HPLC/MS, GC/MS, ICP-MS, and Matrix- assisted Laser Desorption Ionization Mass Spectrometry (MALDI/MS) in their theory and many applications. Importantly, the students will also learn the Fundamentals of Electrochemistry, Electrodes and Potentiometry, Pulsed Amperometry, and other Electrochemical Techniques and their many real-life applications.



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